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Purified Viable Fat Suspended in Matrigel Improves Volume Longevity

Justin Howard Piasecki, Karol A. Gutowski, Katherine M. Moreno, Garet L. Lahvis
DOI: http://dx.doi.org/10.1016/j.asj.2007.09.006 24-32 First published online: 1 January 2008


Background: Autologous fat is an excellent soft tissue filler with cosmetic and reconstructive utility. However, graft longevity is unpredictable.

Objective: This study sought to evaluate the effect on in vivo fat graft performance of contemporary adipocyte tissue engineering techniques that have not previously been applied to mature fat cells due to the difficulty of their purification and their high metabolic demand.

Methods: Using a recently reported protocol, the adipocyte viability and purity of lipo-harvested fat were optimized. Before graft administration, these purified cells were suspended in GFR-Matrigel (BDBiosciences), a basement membrane protein matrix known to improve early angiogenesis. It was posited that by suspending the purified cells in this resorbable matrix, the high metabolic demand of these cells would be met and graft performance could be improved. The in vivo longevity of these tissue engineered fat grafts was tested in a murine model in which each subject received posterior subcutaneous injections of three types of fat graft: unpurified fat after lipo-harvest alone; fat harvested in identical fashion, but purified and suspended in GFR-Matrigel; and a control of GFR-Matrigel alone. Graft volumes and quantitative histologic characteristics were examined at 1 week, 1 month, and 3 months.

Results: At 3 months, purified fal/GFR Matrigel grafts showed superior fat volume maintenance (80.2% versus 29.7% for unpurified grafts [P < .05]) and adipocyte cellular longevity (70.1% versus 45.6% [P < .001). Unpurified grafts were largely replaced by fibrosis at 3 months (96.5% [95% CI 0.90–0.970]), despite starting with three times as many viable adipocytes as purified grafts. A correlation was noted between the poor performance of unpurified grafts and a disproportionate presence of early inflammation in fat grafts prepared without purification techniques.

Conclusions: A preparatory regimen consisting of a preadministration purification followed by cellular suspension in a resorbable protein matrix may ultimately improve the predictability and longevity of autologous fat grafts.

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